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On the Stability of Deinoxanthin Exposed to Mars Conditions during a Long-Term Space Mission and Implications for Biomarker Detection on Other Planets

机译:长期在太空任务中暴露于火星条件下的地黄嘌呤的稳定性及其对其他行星生物标志物检测的意义

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摘要

Outer space, the final frontier, is a hostile and unforgiving place for any form of life\udas we know it. The unique environment of space allows for a close simulation of\udMars surface conditions that cannot be simulated as accurately on the Earth. For this\udexperiment, we tested the resistance of Deinococcus radiodurans to survive exposure\udto simulated Mars-like conditions in low-Earth orbit for a prolonged period of time as part\udof the Biology and Mars experiment (BIOMEX) project. Special focus was placed on the\udintegrity of the carotenoid deinoxanthin, which may serve as a potential biomarker to\udsearch for remnants of life on other planets. Survival was investigated by evaluating\udcolony forming units, damage inflicted to the 16S rRNA gene by quantitative PCR,\udand the integrity and detectability of deinoxanthin by Raman spectroscopy. Exposure\udto space conditions had a strong detrimental effect on the survival of the strains and the\ud16S rRNA integrity, yet results show that deinoxanthin survives exposure to conditions\udas they prevail on Mars. Solar radiation is not only strongly detrimental to the survival\udand 16S rRNA integrity but also to the Raman signal of deinoxanthin. Samples not\udexposed to solar radiation showed only minuscule signs of deterioration. To test whether\uddeinoxanthin is able to withstand the tested parameters without the protection of the\udcell, it was extracted from cell homogenate and exposed to high/low temperatures,\udvacuum, germicidal UV-C radiation, and simulated solar radiation. Results obtained\udby Raman investigations showed a strong resistance of deinoxanthin against outer\udspace and Mars conditions, with the only exception of the exposure to simulated solar\udradiation. Therefore, deinoxanthin proved to be a suitable easily detectable biomarker\udfor the search of Earth-like organic pigment-containing life on other planets.
机译:外太空是最后的疆界,是我们所知道的任何形式的生活\敌对和无情的地方。独特的空间环境允许对\ udMars表面条件进行紧密模拟,而在地球上则无法如此精确地模拟。在这项实验中,作为生物学和火星实验(BIOMEX)项目的一部分,我们测试了辐射杜氏球菌在低地球轨道上模拟的类似火星的条件下长期暴露的抵抗能力。特别关注的是类胡萝卜素去黄嘌呤的\\完整性,这可能是\研究其他星球上剩余生命的潜在生物标记。通过评估\ udcolony形成单位,定量PCR对16S rRNA基因造成的损害,\ demanant黄嘌呤的完整性和可检测性(通过拉曼光谱法)来评估存活率。暴露于太空条件对菌株的存活和\ ud16S rRNA完整性具有强烈的有害影响,但结果表明去黄嘌呤在暴露于火星的条件下仍能幸存。太阳辐射不仅严重损害存活率和16S rRNA的完整性,而且还严重损害去黄嘌呤的拉曼信号。未\暴露于太阳辐射的样品仅显示出微小的劣化迹象。为了测试\ uddeinoxanthin是否能够在没有保护\ udcell的情况下承受测试的参数,将其从细胞匀浆中提取并暴露于高温/低温,\ udvacuum,杀菌UV-C辐射和模拟太阳辐射下。拉曼研究获得的结果表明,除黄嘌呤对外层空间和火星条件具有很强的抵抗力,唯一的例外是暴露于模拟的日光辐射。因此,事实证明去黄嘌呤是一种合适的易于检测的生物标志物,可用于寻找其他行星上类似地球的有机颜料。

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